Review



trna shrna expression vector  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    TaKaRa trna shrna expression vector
    Trna Shrna Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trna+shrna+expression+vector/us10383925-441-73-79
    Average 86 stars, based on 1 article reviews
    trna shrna expression vector - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    shRNA:

    Article Title: Cancer antigen EEF2
    Article Snippet: .. Construction of ShRNA In order to construct an shRNA for the above sequences (SEQ ID NOs:18 and 19), a DNA sequence [shEF-1918 or shEF-2804 (sense strand)] consisting of a sense sequence of a target sequence (30 bases)—a loop sequence (10 bases)—an antisense sequence (30 bases), and its complementary DNA sequence [shEF-1918 or shEF-2804 (antisense strand)] were chemically synthesized and then annealed, and the product was inserted into SacI and KpnI recognition sites of tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). ..

    Article Title: Cancer antigen eEF2
    Article Snippet: .. Construction of ShRNA In order to construct an shRNA for the above sequences (SEQ ID NOs:18 and 19), a DNA sequence [shEF-1918 or shEF-2804 (sense strand)] consisting of a sense sequence of a target sequence (30 bases)−a loop sequence (10 bases)−an antisense sequence (30 bases), and its complementary DNA sequence [shEF-1918 or shEF-2804 (antisense strand)] were chemically synthesized and then annealed, and the product was inserted into SacI and KpnI recognition sites of tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). ..

    Construct:

    Article Title: Cancer antigen EEF2
    Article Snippet: .. Construction of ShRNA In order to construct an shRNA for the above sequences (SEQ ID NOs:18 and 19), a DNA sequence [shEF-1918 or shEF-2804 (sense strand)] consisting of a sense sequence of a target sequence (30 bases)—a loop sequence (10 bases)—an antisense sequence (30 bases), and its complementary DNA sequence [shEF-1918 or shEF-2804 (antisense strand)] were chemically synthesized and then annealed, and the product was inserted into SacI and KpnI recognition sites of tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). ..

    Article Title: Cancer antigen eEF2
    Article Snippet: .. Construction of ShRNA In order to construct an shRNA for the above sequences (SEQ ID NOs:18 and 19), a DNA sequence [shEF-1918 or shEF-2804 (sense strand)] consisting of a sense sequence of a target sequence (30 bases)−a loop sequence (10 bases)−an antisense sequence (30 bases), and its complementary DNA sequence [shEF-1918 or shEF-2804 (antisense strand)] were chemically synthesized and then annealed, and the product was inserted into SacI and KpnI recognition sites of tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). ..

    Sequencing:

    Article Title: Cancer antigen EEF2
    Article Snippet: .. Construction of ShRNA In order to construct an shRNA for the above sequences (SEQ ID NOs:18 and 19), a DNA sequence [shEF-1918 or shEF-2804 (sense strand)] consisting of a sense sequence of a target sequence (30 bases)—a loop sequence (10 bases)—an antisense sequence (30 bases), and its complementary DNA sequence [shEF-1918 or shEF-2804 (antisense strand)] were chemically synthesized and then annealed, and the product was inserted into SacI and KpnI recognition sites of tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). ..

    Article Title: Cancer antigen eEF2
    Article Snippet: .. A vector expressing a siRNA which targets at sequence: 5′-caugggcaacaucaugaucgauccuguccu-3′ of an eEF2 mRNA (hereinafter, referred to as shEF2) was prepared using a tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). .. Subsequently, 10 μg of shEF2 or a vacant shRNA vector (shMock) was introduced by electroporation into stomach cancer cell lines AZ-521 and MKN28, colon cancer cell line SW620, lung cancer cell lines LU99B and PC-14, pancreatic cancer cell lines MiaPaCa2 and PCI6, glioblastoma cell lines A172 and U87MG, as well as malignant lymphoma cell lines 134 and YT (each 5×105 cells) expressing the eEF2 using Gene Pulser Xcell (trademark) system (Bio Rad, Hercules, Calif.) under a condition of 165 V and 1000 μF.

    Article Title: Cancer antigen eEF2
    Article Snippet: .. Construction of ShRNA In order to construct an shRNA for the above sequences (SEQ ID NOs:18 and 19), a DNA sequence [shEF-1918 or shEF-2804 (sense strand)] consisting of a sense sequence of a target sequence (30 bases)−a loop sequence (10 bases)−an antisense sequence (30 bases), and its complementary DNA sequence [shEF-1918 or shEF-2804 (antisense strand)] were chemically synthesized and then annealed, and the product was inserted into SacI and KpnI recognition sites of tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). ..

    Synthesized:

    Article Title: Cancer antigen EEF2
    Article Snippet: .. Construction of ShRNA In order to construct an shRNA for the above sequences (SEQ ID NOs:18 and 19), a DNA sequence [shEF-1918 or shEF-2804 (sense strand)] consisting of a sense sequence of a target sequence (30 bases)—a loop sequence (10 bases)—an antisense sequence (30 bases), and its complementary DNA sequence [shEF-1918 or shEF-2804 (antisense strand)] were chemically synthesized and then annealed, and the product was inserted into SacI and KpnI recognition sites of tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). ..

    Article Title: Cancer antigen eEF2
    Article Snippet: .. Construction of ShRNA In order to construct an shRNA for the above sequences (SEQ ID NOs:18 and 19), a DNA sequence [shEF-1918 or shEF-2804 (sense strand)] consisting of a sense sequence of a target sequence (30 bases)−a loop sequence (10 bases)−an antisense sequence (30 bases), and its complementary DNA sequence [shEF-1918 or shEF-2804 (antisense strand)] were chemically synthesized and then annealed, and the product was inserted into SacI and KpnI recognition sites of tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). ..

    Expressing:

    Article Title: Cancer antigen EEF2
    Article Snippet: .. Construction of ShRNA In order to construct an shRNA for the above sequences (SEQ ID NOs:18 and 19), a DNA sequence [shEF-1918 or shEF-2804 (sense strand)] consisting of a sense sequence of a target sequence (30 bases)—a loop sequence (10 bases)—an antisense sequence (30 bases), and its complementary DNA sequence [shEF-1918 or shEF-2804 (antisense strand)] were chemically synthesized and then annealed, and the product was inserted into SacI and KpnI recognition sites of tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). ..

    Article Title: Cancer antigen eEF2
    Article Snippet: .. A vector expressing a siRNA which targets at sequence: 5′-caugggcaacaucaugaucgauccuguccu-3′ of an eEF2 mRNA (hereinafter, referred to as shEF2) was prepared using a tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). .. Subsequently, 10 μg of shEF2 or a vacant shRNA vector (shMock) was introduced by electroporation into stomach cancer cell lines AZ-521 and MKN28, colon cancer cell line SW620, lung cancer cell lines LU99B and PC-14, pancreatic cancer cell lines MiaPaCa2 and PCI6, glioblastoma cell lines A172 and U87MG, as well as malignant lymphoma cell lines 134 and YT (each 5×105 cells) expressing the eEF2 using Gene Pulser Xcell (trademark) system (Bio Rad, Hercules, Calif.) under a condition of 165 V and 1000 μF.

    Article Title: Cancer antigen eEF2
    Article Snippet: Also, the shRNA or siRNA of the present invention may be prepared in the form of an shRNA expression vector or a siRNA expression vector containing a DNA sequence as shown in the above SEQ ID NO:20 or 22. .. In this case, for example, tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.) may be used for the preparation. ..

    Article Title: Cancer antigen eEF2
    Article Snippet: .. Construction of ShRNA In order to construct an shRNA for the above sequences (SEQ ID NOs:18 and 19), a DNA sequence [shEF-1918 or shEF-2804 (sense strand)] consisting of a sense sequence of a target sequence (30 bases)−a loop sequence (10 bases)−an antisense sequence (30 bases), and its complementary DNA sequence [shEF-1918 or shEF-2804 (antisense strand)] were chemically synthesized and then annealed, and the product was inserted into SacI and KpnI recognition sites of tRNA-shRNA expression vector, piGENE tRNA Pur (Clontech, Palo Alto, Calif.). ..



    Similar Products

    86
    TaKaRa trna shrna expression vector
    Trna Shrna Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trna+shrna+expression+vector/us10383925-441-73-79
    Average 86 stars, based on 1 article reviews
    trna shrna expression vector - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    90
    OriGene lars1 shrna expression vector
    Lars1 Shrna Expression Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trna+shrna+expression+vector/Leucyl+tRNA+synthetase+(LARS)+Human+shRNA+Plasmid+Kit/pm36612155-109-18-23
    Average 90 stars, based on 1 article reviews
    lars1 shrna expression vector - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Igene Biotechnology Inc shrna expression vectors pigene tm trna neo/kpni/saci
    Shrna Expression Vectors Pigene Tm Trna Neo/Kpni/Saci, supplied by Igene Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trna+shrna+expression+vector/shrna+expression+vectors+pigene+tm+trna+neo+kpni+saci/pmc02740444-83-3-14
    Average 90 stars, based on 1 article reviews
    shrna expression vectors pigene tm trna neo/kpni/saci - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    TaKaRa trna shrna expression vector pigene trna pur
    Trna Shrna Expression Vector Pigene Trna Pur, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trna+shrna+expression+vector/pmCherry+Vector/pm16630117-29-41-47
    Average 96 stars, based on 1 article reviews
    trna shrna expression vector pigene trna pur - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results